Retention and resolution
Optimize chromatographic conditions when compounds are poorly retained or insufficiently separated.
Analytical development
HPLC/UPLC Method Development and Validation
Chromatographic method development, optimization and validation to support pharmaceutical characterization, assay determination, impurity analysis and stability investigations.
Analytical methods designed around the question
A useful analytical method needs to be appropriate for the product, analyte and intended purpose. Phanso develops and optimizes HPLC and UPLC methods to address practical analytical requirements and investigate difficult chromatographic behaviour.
Depending on the project, method development may involve selection of chromatographic conditions, optimization of retention and resolution, evaluation of peak shape, sample preparation, detection wavelength and robustness-related parameters.
Analytical services
- HPLC and UPLC method development and optimization
- Assay and content determination
- Impurity and degradation-product analysis
- Stability-indicating method development
- Investigation of peak shape, resolution, pressure or reproducibility issues
- Analytical method validation
Common analytical challenges
Phanso can help investigate problems that affect method performance or interpretation of results.
Peak shape and pressure
Investigate tailing, broadening, rising backpressure and other chromatographic symptoms.
Assay and recovery
Review sample preparation, recovery and analytical response when results are unexpected.
Stability-indicating analysis
Develop methods to help assess the active ingredient alongside relevant degradation-related changes.
Need to develop or troubleshoot an HPLC method?
Share the analyte, dosage form, current method and the issue you are seeing.